Production of Xylanase by Trichoderma sp. Via ...

Xylanase (E.C 3.2.1.8) is a wide spread group of enzyme which can catalyse the endohydrolysis of 1,4-β-D-xylosidic linkages in xylan. It has a wide range of application in industrial areas. Trichoderma sp. was selected as a potential producer of xylanase via a solid state fermentation system (SSF) using sugarcane bagasse as substrate. Fermentation was proceeded in the condition of 5.6 g sugarcane bagasse, 1% sucrose (w/w), 1% yeast extract as solid substrate and 70% moisture content (v/w). The aim of this study was to identify the ideal physical conditions of SSF for the highest xylanase acitivity. Thus, different fermentation days, concentration of carbon sources, moisture content and pre-incubation temperatures to the medium were conducted. The results obtained show that the higher xylanase enzyme activity of 380 U/g substrate DW was the maximum activity by using 1% sucrose as carbon source, 50oC of pre-incubation temperature, 70% (v/w) of moisture content at the fermentation day of six.