Effect of Poly (Toluene Sulphonic Acid) in ...

The effect of poly(tolune sulphonic acid) in enhancing durability of (poly)pyrrole/(poly)N-methyl pyrrole/ glucose oxidase sensor has been reported in the present investigation. The (poly) pyrrole/(poly)N-methyl pyrrole composite matrix was synthesized by galvanostatic technique. The poly(toluene sulphonic acid) was added as dopant to enhance susceptibility of the synthesized matrix towards humidity. The polypyrrole – poly (n-methylpyrrole) composite films were subjected to spectral and morphological characterizations. Excellent morphology, suitable for immobilization of glucose–oxidase, was achieved with optimized process parameters. The glucose–oxidase was immobilized on the composite matrix by cross linking via glutaraldehyde. Comparative study was carried out to investigate functionality of the sensor in phosphate and acetate buffer atmosphere. The biosensor exhibited an excellent linear response for a wide range of glucose concentration from 0mM to 10mM in both the buffers. However the sensitivity of the developed biosensor in presence of phosphate buffer (0.41 µA/mM) was found to be higher. The durability of the sensor was found to be excellent (47 days) in presence of poly (toluene sulphonic acid) in phosphate buffer.