Use of Partially Purified Banana Peel ...

Polyphenol oxidase (PPO) enzyme was isolated from banana peel. Enzyme was partially purified by ammonium sulphate precipitation (70%). The partially purified PPO showed optimum temperature 35 oC and pH 7.0. This PPO enzyme preparation was employed for the degradation of four different phenolic compounds and textile dye Blue 2RNL. PPO degraded 1 g/L gallic acid (100%) in 24 h. PPO also decolorized 40 mg/L Blue 2RNL dye (89%) in 24 h. The decolorization of Blue 2RNL dye by PPO enzyme was evaluated and confirmed by UV-Vis spectroscopy analysis. The treatment of phenolic compounds and textile dye Blue 2RNL with PPO resulted in the remarkable loss of chemical oxygen demand (COD). Also the degradation products showed no toxicity for germination of various plant seeds.